Description

Liraglutide (LRT) ELISA Kit is an ELISA kit for the quantitative in vitro measurement of liraglutide (LRT) concentrations in serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.

Introduction

Liraglutide (NN2211) is a derivative of human incretin (metabolic hormone) glucagon-like peptide 1 (GLP-1) that is used as a long-acting glucagon-like peptide 1 receptor agonist, which binds to glucagon receptors than the endogenous metabolic hormone GLP-1 that stimulates insulin secretion.

Destination

Liraglutide (LRT)

Reactivity

General (all species)

Proven applications

ELISA

Recommended dilutions

The end-user must determine the optimal dilutions/concentrations.

Storage

Shipped at 4 ° C. Upon receipt, store the kit according to the storage instructions in the kit manual.

Validity

The validity of this kit is 6 months.

Stability

The stability of the kit is determined by the rate of loss of activity. The loss rate is less than 5% within the expiration date under proper storage conditions. To minimize fluctuations in performance, operating procedures and laboratory conditions must be strictly controlled. It is also strongly suggested that all testing be performed by the same user at all times.

Test range

12.35 pg / ml – 1000 pg / ml

Sensitivity

<4.64 pg / ml

Standard form

Lyophilized

Detection method

Colorimetric

Sample type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids.

Target-type

Antigen

Assay Principle

This kit is based on competitive enzyme-linked immunosorbent assay technology. An antibody is precoated in a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. A competitive inhibition reaction occurs between the biotin-labeled LRT and the unlabeled LRT on the pre-coated antibody. The HRP-conjugated reagent is then added and the entire plate is incubated. Unbound conjugates are removed using a wash buffer at each step.

The TMB substrate is used to quantify the HRP enzymatic reaction. After adding TMB Substrate, only wells containing sufficient LRT will produce a blue product, which then turns yellow after adding Acid Stop Solution. The intensity of the yellow color is inversely proportional to the amount of LRT bound to the plate. OD is measured spectrophotometrically at 450 nm in a microplate reader, from which the LRT concentration can be calculated.

Availability

Shipped within 5-7 business days.

Note

This product is for research only. Range and sensitivity are subject to change. Contact us for the latest product information. For accurate results, sample concentrations must be diluted to half the kit range. If you need a specific range, please contact us in advance or write your request in the comments of your order. Please note that our ELISA and CLIA kits are optimized for the detection of native samples, rather than recombinant proteins. We cannot guarantee the detection of recombinant proteins, as they may have different sequences or tertiary structures than the native protein.

Standard

1000 pg / ml